Calculated Factors:

Whitlock S.F.E.L.O 4 X 0.5ml slide

The mean of two counts is recommended in calculating egg counts.
(click here to see the equation and general calculations to calculate eggs per gram from a worm egg count) 
  For 2g faeces in 48ml of (flotation or sedimentation) solution (2g:50ml)
 
 1 strip
2  strips
4  strips
 1 chamber
=5 strips		 2 chambers 4 chambers
 Eggs counted in
0.01ml
0.02ml
0.04ml
0.5 ml
1.0 ml
2.0 ml
Dilution
2g:50ml
 
 
 
 
 
 
 
FACTOR x
250
125
62.5
50
25
12.5
1:25 *
.
.
 
 
.
.
.
.
.
For other dilutions:
 
 
 
 
 
 
1.5g:60ml x
400
200
100
80
40
20
1:40
2g:60ml x
300
150
75
60
30
15
1:30
3g:60ml x
200
100
50
40
20
10
1:20
4g:60ml x
150
75
37.5
30
15
7.5
1:15
1g:50ml
500
250
125
100
50
25
1:50
* recommended generic dilution- para 9.1 of reference  for simplest calculations

Reference:
Details about the techniques which use these slides may be found in the Section on Anthelmintic Resistance in Sheep (para 9.1 in particular) in
Australian Standard Diagnostic Techniques for Animal Diseases.  - ISBN 0 643 05243 7     636.0896075
developed through the Standing Committee on Agriculture and Resource Management,  Animal Health Committee,
Sub-committee on Animal Health Laboratory Standards
and published on their behalf by CSIRO Information Services, Australia.
Available on our website at  www.whitlock.com.au/slides/pdf/ASDT-AnthrlmResist-signed.pdf
and on the SCAHLS website at http://www.scahls.org.au/asdts/05-AnthelminiticResistanceinSheep.pdf
approx 1.6Mb files

(Note that the recommended dilution is intended for the Whitlock Universal slide which is the basis of the SFELO, to give the simple Factor as above. Alternatives are permitted)

select here for 
Last Update: 31 Oct 2007