Calculated Factors:
Whitlock McMaster 3 X 0.3ml slide
The mean of two counts is recommended in calculating egg counts.
(click here to see the equation and general calculations
to calculate eggs per gram from a worm egg count)
For
2g faeces in 58ml of flotation solution (2g:60ml)
|
Eggs
|
1 strip |
4 strips |
1chamber |
2 chambers |
3 chambers |
|
|
counted in
|
0.0375 ml
|
0.15 ml
|
0.3 ml
|
0.6 ml
|
0.9 ml
|
. |
|
2g:60ml
|
|
|
|
|
|
Dilution
|
| FACTOR x |
800 |
200 |
100 |
50 |
33 |
1:30 |
|
.
|
. |
. |
. |
. |
. |
. |
| . |
For other dilutions: |
|
|
|
|
|
|
2g:50ml x
|
667
|
167
|
83
|
42
|
28
|
1:25 *
|
|
4g:60ml x
|
400 |
100 |
50 |
25 |
17 |
1:15 |
* recommended generic dilution-
para 9.1 of reference.
CSIRO Animal Production use
2gm:60ml total solution for simplest calculations
Reference:
Details about the techniques
which use these slides may be found in the Section on Anthelmintic Resistance
in Sheep (para 9.1 in particular) in
Australian Standard Diagnostic
Techniques for Animal Diseases. - ISBN 0 643 05243 7
636.0896075
developed through the Standing
Committee on Agriculture and Resource Management, Animal Health Committee,
Sub-committee on Animal Health
Laboratory Standards
and published on their behalf
by CSIRO Information Services, Australia.
(Note that the recommended dilution is intended
for the Whitlock Universal slide to give
the simple Factor as above. Alternatives are permitted)
select here for
Last Update: 16/06/99