The overall methods which utilise this information can
estimate the
degree of infestation in livestock and the efficacy of treatments.

**STANDARD
CALCULATIONS:**

download a compressed (24K ZIP)
**FREE** Excel (4
or later) spreadsheet

calculation
program to get you started

A bit of practice looking down the microscope

FREE flash egg counting demonstration

and as a ZIP compressed file

(Note that a lot of antivirus software may try to block this).

The mean of two counts is recommended in calculating egg counts.
A bit of practice looking down the microscope

FREE flash egg counting demonstration

and as a ZIP compressed file

(Note that a lot of antivirus software may try to block this).

To determine the number of eggs per gram **(epg)**
of faeces-

multiplythe number of eggs of each type counted in the scanned area of the slide by

(Volume of faeces sample= weight of faeces sample)

(Measurement volume used may be only a part of the slide)

place2gfaeces in2.5mlwater (or 0.1% aqueous methylene blue) and55.5mlflotation solution and measure or count inone chamber(0.3ml for theWhitlock McMaster)

-(for calculations for other Whitlock slides and different dilutions see Universal , McMaster or Paracytometer):

- (or click here to download a FREE Excel spreadsheet calculation program to get you started)

epg= #eggs * (2.5+2+55.5) / (2* 1*0.3) = # *100where " * " means "multiply"

(in other words,in this instance, multiply the number of eggs counted by 100 to give the.)epg

*Reference:*

Details
about the techniques
which use these slides may be found in the Section on Anthelmintic
Resistance
in Sheep (para 9.1 in particular) in ** Australian
Standard Diagnostic
Techniques for Animal Diseases**. - ISBN 0 643 05243
7 636.0896075
developed through the Standing Committee on Agriculture and Resource
Management,
Animal Health Committee, [SCARM] Sub-committee on Animal Health
Laboratory Standards
[SCAHLS] and published on their behalf by CSIRO Information Services,
Australia.

(Note that the recommended dilution is intended for the Whitlock Universal slide to give a simple Factor as above. Alternatives are permitted)

Available
as 1.6Mb pdf on our website at www.whitlock.com.au/slides/pdf/ASDT-AnthrlmResist-signed.pdf

and on the SCAHLS
website at
http://www.scahls.org.au/asdts/05-AnthelminiticResistanceinSheep.pdf

PO Box 51, EASTWOOD NSW 2122, AUSTRALIA

slides@whitlock.com.au websitephone (02) 9638 1142

international 61 2 9638 1142